Bubble Actuator Enhanced DNA Micro Biosensor

 

Peigang DENG, Yi-Kuen LEE and Ping CHENG

 

 

This paper reports a novel bubble actuator enhanced DNA micro biosensor. The hybrid PDMS silicon micro biosensor chip was fabricated and characterized to dramatically increase the efficiency of DNA biosensing using molecular beacon technology.

 

 

Keywords: micro bubble; molecular beacon; biosensor

 

 

ACKNOWLEDGEMENTS

 

This work was supported by the Hong Kong Research Grant Council (HKUST6134/04E) and a HKUST PDF grant.

 

 

REFERENCES

 

[1]  D. Ivnitski, I. Abdel-Hamid, P. Atanasov, and  E. Wilkins, “Biosensors for detection of pathogenic bacteria,” Biosen. Bioelectron., vol. 14, 

      pp. 599–624, Oct. 1999.

[2]  M. L. M. Anderson, Nucleic Acid Hybridization, New York: Bios Scientific Publishers, 1999.

[3] K. Pappaert et al., “Enhancement of DNA micro-array analysis using a shear-driven micro-channel flow system,” J. Chromatogr. A, vol. 1014,

     pp. 1-9, Oct. 2003.

[4] S. Tyagi and F. R. Kramer, “Molecular Beacons: Probes that Fluoresce upon Hybridization,” Nat. Biotechnol., vol. 14, pp. 303-308, Mar. 1996.

[5] A. Tsourkas, M. A. Behlke, S. D. Rose, and G. Bao, “Hybridization kinetics and thermodynamics of molecular beacons,” Nucleic Acids Res., vol. 31,

     pp. 1319-1330, Feb. 2003.

[6] IDT OligoAnalyzer 3.0, http://207.32.43.70/biotools/oligocalc/ oligocalc.asp.

[7]  P. Deng, Y.-K. Lee and P. Cheng, “The growth and collapse of a micro-bubble under pulse heating,” Int. J. Heat Mass Tran., vol. 46, pp. 4041-4050,

      Oct. 2003.

[8] P. Deng, Y.-K. Lee and P. Cheng, “Micro Bubble Dynamics in DNA solutions,”  J. Micromech. Microeng., vol. 14, pp. 693-701, May. 2004.

[9] P. Deng, Y.-K. Lee and P. Cheng, “Measurements of Micro Bubble Nucleation Temperatures in DNA Solution,” J. Micromech. Microeng., vol. 15,

     pp.564-574, Mar. 2005.